Evaluation of TSLP promoter polymorphism between ectopic tissue and peripheral blood in endometriosis patients

Background: Endometriosis is a chronic inflammatory disease characterized by the presence of tissue resembling the endometrium. By aberrant production of cytokines, immune dysregulation has been proposed as a mechanism in endometriosis. Thymic Stromal Lymphopoietin (TSLP) is a critical cytokine that one single-nucleotide polymorphism (SNP), C-847T, in the promoter region of this gene creates a binding site for the transcription factor activating protein (AP)–1 subsequently increases expression of TSLP. The present study aimed to evaluate the frequency of TSLP promoter polymorphism in endometrioma tissue and comparing it to peripheral blood in endometriosis patients.Methods: This study was conducted on 28 endometriosis patients diagnosed by laparoscopy and confirmed with pathological test at Royan Institute. PCR-sequencing was done on both extracted DNA samples from endometriotic tissues and peripheral blood of patients. The statistical analysis was done by Chi Square (PResult: In women with endometriosis, the mean of age and Body Mass Index (BMI) were 30.34±4.34 years old; 23.56±2.79 kg/m2, respectively. Although the CC genotype wasn t detected in both groups, CT and TT genotypes were observed to be 78.5% and 21.5% in DNA of endometriotic tissue, whereas these frequencies were 64.5% and 35.5% in DNA extracted from whole blood.Conclusion: Our results showed that the frequency of C-847T genotypes is altered between ectopic tissue and peripheral blood; however, larger sample size studies is essential. It seems the assessment of polymorphism in ectopic tissue could create a better view for influencing SNP in etiology of endometriosis.

کلیدواژه ها:

Polymorphism ، Thymic stromal lymphopoietin ، TSLP ، Endometriosis