Recurrent spontaneous abortion (RSA) is associated with maternal KIR2DS1 in combination with paternal HLA-C2 gene: A case-control study

Background: Among the approaches to recurrent spontaneous abortion (RSA), immune system and its involving molecules can be pointed out. Natural killer cells (NKs) are the most important cells in the fetomaternal immune tolerance inducing through interaction of maternal killer-cell immunoglobulin-like receptors (KIR) and fetal human leucocyte antigens (HLA). Hence, we intended to investigate maternal KIR, maternal and paternal HLA-C, and maternal-paternal KIR-HLA interaction in both RSA and control groups.Methods: For the current case-control study in Khorramabad, 200 couples participated. DNA samples were genotyped based on polymerase chain reaction with specific sequences of primers (PCR-SSP) assay. The significance of associations were determined using the χ2 test with Yate s correction (or Fisher s exact test if necessary) in 2 by 2 tables for each KIR gene. This study has been approved by the ethic committee of Lorestan University of Medical Sciences with registration number lums.rec.1394,10 .Result: Among the maternal KIR genes, maternal HLA-C genes, and maternal KIR-HLA combinations, no significant deference was observed between the cases and controls. A significant relation was found for maternal KIR and paternal HLA-C combination. The relation was for the activating combination KIR2DS1+HLA-C2 in the RSA group (P=0.008; OR=2.27).Conclusion: The activating combinations of KIR-HLA seems to be more associated with RSA. Prediction of RSA with help of maternal KIR typing and paternal HLA-C typing can be possible in future.

کلیدواژه ها:

Abortion ، HLA-C ، PCR-SSP ، KIR