Effect of royal jelly on the relative expression of GDF-9 BMP-15 genes on in vitro maturation of sheep oocytes

Background: This study was conducted o investigate the different percentages of royal jelly on in vitro maturation of sheep oocyte.Methods: Sheep ovaries were collected from local slaughter house and transferred to the laboratory via term-flask containing warm saline (35˚C) with 100 IU/ml of penicillin. COCs were recovered from antralfollicles (2-6 mm) by slicing method and cultured in to the medium 199 containing 10% FBS, 0.01 U/ml o FSH, 0.2 mM pyruvate and 0.2 mM glutamine and in a different percentages of royal jelly (0 , 0.25 , 0.5 and 1%). COCs cultured in maturation medium reached to MII after 24 hours. Data were analyzed as a compeletly randomized design with 4 treatments and 4 replicate in each.Result: The results of this study demonstrated that the addition of royal jelly to medium increased the rate of oocyte maturation (p Conclusion: The results indicated that the increase in percentages of different royal jelly increased significantly in vitro maturation oocyte rate from the control (61%) than the one percent(89%) in (p

کلیدواژه ها:

BMP15 ، GDF9 ، Oocyte cumulus complex ، Sheep ، Royal jelly