Comparison of extra virgin olive oil and soybean oil in maternal isocaloric diet on transcriptional and osteogenic markers in bone of female mice offspring at adolescence

Background: Recently, there is a new insight into the transcriptional regulation of osteogenic differentiation like runt-related gene 2 (Runx2), as well as osteogenic markers such as collagens (COLs), alkaline phosphatase (Alk.ph) specific to each stage of the osteoblast life cycle. Maternal diet in fetal early growth (in utero and early postnatal life) predicts reduced bone mass, and so risk of fracture in later life. In the present study, we compared the effects of low and high amounts of extra virgin olive oil (EVOO), in an isocaloric diet, during gestation and lactation on gene expression of transcriptional and osteogenic markers in adolescent female mice offspring.Methods: Virgin female C57BL/6 mice were impregnated and fed either the AIN 93G diet (received 16% of calorie as soybean oil [LFS] or EVOO [LFO]) or a high fat AIN 93G diet (received 45% of calorie as soybean oil [HFS] or EVOO [HFO]) from the time of vaginal plug confirmation until the offspring was weaned. Offspring were euthanized at 6 weeks and genes expression was measured by real time-PCR from the right femur.Result: Runx2, Alk.ph and COLI were significantly higher in the offspring born from LFO than LFS, HFO than HFS and LFO than HFO-fed mothers (pConclusion: Maternal EVOO consumption during gestation and lactation lead to increase in transcriptional and osteogenic markers in bone of female mice offspring at adolescence.

کلیدواژه ها:

Dietary oil ، Fetal programming ، Gestation ، Lactation ، Osteoblastogenesis