The effect of growth factors on proliferation and viability of chicken spermatogonial stem cells

Background: The aim of this study was to evaluate the effect of the different growth factors on growth, proliferation, and viability of chicken spermatogonial stem cells (SSCs) in vitro culture.Methods: The SSCs isolated from 4-6 week-old Arbor Acres chickens testes. It was cultured for 7 days with DMEM medium in four different treatments groups of growth factors including: 1) leukemia inhibitory factor (LIF) , basic fibroblast growth factor (bFGF) and glial cell derived neurotrophic factor (GDNF). 2) GDNF, bFGF. 3) GDNF. 4) FBS 10% without any growth factors. The colonies were assessed at 7th day of culture.Result: The result of immunocytochemistry showed that cell colonies were positive for the three SSC specific markers SSEA1, SSEA3, vasa. Also, PAS test was positive for all treatment groups. The colony numbers, and cells/colony in treatment group No. (1) were significantly higher than other groups when compared with others (p≤0.01).Conclusion: Results of this study show that growth factors can improve the growth and proliferation of chicken SSCs, but the synergic effect of three growth factors including, GDNF, LIF and bFGF have the most positive effect on chicken SSCs during in vitro culture.

کلیدواژه ها:

Chicken ، Growth factors ، Spermatogonial stem cells