Study of Changes in Bax and Bcl2 Genes Experssion in HT-29 Cell Exposed with Lactobacillus Rhamnosus PTCC1637
محل انتشار: اولین کنفرانس ملی یافته های نوین زیست شناسی
سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 282
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شناسه ملی سند علمی:
BIOC01_182
تاریخ نمایه سازی: 11 خرداد 1397
چکیده مقاله:
In the present experimental study, the toxic effects of extracts of Lactobacillus Rhamnosus PTCC1637 on colon cancer cells (HT-29) were assayed at concentrations of 0.01, 0.1, 1,10,100,1000, and 10 µg /ml of extract using MTT assay.Also HT-29 cells were cultured in RPMI-1640 medium. Data were analyzed by one-way ANOVA method(P<0.05) . Then genes experssion chenges of Bax and Bcl2 were assessed by Real Time PCR . Our findings showed that administration 1up to 1000 µg /ml in 48 hours of Lactobacillus Rhamnosus PTCC1637 extract resulted in significant decreased viability of HT-29 cells in cell culture. According to our finding, chenges in genes expersion Bax and Bcl2 respectively indicated increasing and lowering
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نویسندگان
Masoum Abniki
Msc of Biotechnology , Majoring in Microbial, Islamic Azad University Damghan Branch, Damghan, Iran
Fatemeh Ashrafi
Assistant Professor of Microbiology, Academic member of Islamic Azad University, North Tehran Branch,Tehran, Iran.