Prevalence of methicillin resistance gene (mecA) in Staphylococcus aureus strains isolated from clinical samples from Imam Khomeini hospital using polymerase chain reaction

lipase biocatalysts are important reactions in aqueous medium and the valuable time doing non-aqueous en-vironment. Among the lipases of plant, animal and microbial microbial lipases are most widely used. Because germs can easily be cultured and lipases derived from them can easily range of hydrolytic and synthetic reac-tions they catalyze. Lipase in the broad fields of Biotechnology, especially dairy (cheese ripening, improvement and development of flavor, enzyme modified cheese production EMC), detergents, drugs (ibuprofen, naprox-en), chemicals, agriculture (pesticides, insecticides ) and oil chemistry (hydrolysis of fats and oils, synthetic bio-surfactant) are used. And in the coming years in many new fields will be used as a potential biocatalysts. Due to the low cost of extraction of fungal lipases, and resistance to pH and temperature, substrate selectivity and activity in organic solvents as compared to bacterial lipases used more. the sample was transferred to the me-dium of PDA and YGC. In this stage, 20 isolates had the ability to produce lipase. After extraction of ribosomal DNA (18 sRNA) and perform PCR enzymes spectrophotometric quantitation was at a wavelength of 410 Nano-meter. Penicillium chrysogenum having the highest lipase enzymes for molecular identification and sequencing were sent to the laboratory microgene South Korea. Molecular identification of fungi isolated from the Peni-cillium chrysogenum strain of KC884440.1 assignment was approved. High fat and increased lipase enzyme in the body can have complications such as pancreatitis. Research results Mr Nasrallah said in 20140 that genes encoding the lipase and lipase secretion of virulence factors in microorganisms optimize production conditions of temperature and pH were done with two parameters. The doctor Shadzi in Medical Mycology (SID of 1379, p. 328) and fungal infections opportunistic in people with immune deficiency writes: If the fungus to body temperature susceptible host (humans 37 ° C) to bear and able to produce the necessary enzymes and toxins to destroy tissue, can be considered pathogenic

کلیدواژه ها:

Staphylococcus aureus ، an antibiotic resistance gene mec A ، polymerase chain reaction