miR-590 upregulates the NF-kB expression in breast cancer cell lines, MCF-7 and MDA-MB-231

Introduction: Breast cancer is a heterogenous disease which is considered as the most common malignancy that develops in women worldwide. Despite all efforts on identifying cancer, there is no definite therapy yet and much more attempts in discovering cancer biology seems necessary. Immunology of tumors declares the relevance of immune system, chronic inflammation and cancer. Several studies indicate that the immune system may either aid in the prevention as well as the promotion of carcinogenesis. Materials & Methods: Evaluating the progressing activity of immune system and inflammation in many diseases, we discovered some molecular signalingpathways common in inflammation and cancer and then detected the microRNAs which play pivotal roles in mediating these pathways. Using bioinformatic assays, signaling pathways common in both inflammation and cancer, and microRNAs mediating them were detected. miR-590 was selected and cloned into the pLenty-III-eGFP vector and then transfected into the breast cancer cell lines, MCF-7 and MDA-MB-231 by means of XtremeGENE HP DNA Transfection Reagent. Results: 72 hours aftertransfection, the expression levels of miR-590 and NF-kB were measeured by Real-Time PCR. miR-590 was over-expressed as it was expected to occur, whereas, NF-kB level was increased unexpectedly. Conclusion: Although, miR-590 was selected as a microRNA which triggers and downregulates the molecules and genes of signaling pathways similar in cancer and inflammation such as NF-kB, a significant increase was observed in NF kB which may be due to the sudden entry of the vector into the cells which simulate the acute inflammation condition. In fact, more studies are needed to investigate the miR-590 function on cancer and inflammation.

کلیدواژه ها:

Breast cancer ، Inflammation ، microRNAs ، NF-kB