The Effect of Thymoquinone on Level of FOXM1 mRNA in Glioblastoma

Introduction and Objective: Glioblastoma is the most common type of malignant brain tumor. Since the tumors contain different types of cells, the treatment of glioblastoma is difficult. Thymoquinone (TQ), the main active constituent of Nigella sativa s seeds, is one of the drugs that exhibits anticancer characteristics. The aim of this study was to investigate the effect of thymoquinone on the levels of foxm1 mRNA in U87MG glioblastoma cell line.Material/Method: U87MG cells were treated with or without thymoquinone (50µM).The effect of thymoquinone on the expression of foxm1 was analysed by semi-quantitative polymerase chain reaction (Semiq-PCR). TBP was used as housekeeping gene.Results: TQ (50µM) is able to significantly reduce the mRNA levels of foxm1 after 20 hrs treatment. Discussion and result: According our results, TQ can affect the expression of foxm1 gene that involved in tumorigenesis and cancer progression. Therefore, TQ can be suggested as an effective drug for glioblastoma treatment.

کلیدواژه ها:

Thymoquinone ، glioblastoma ، foxm1 ، anticancer drug