Effect of Coronopilin-loaded NIPAAm-MAA nanoparticle onT47D cell line

Cancer is defined as an uncontrolled proliferation of clonally derived cells. Breast cancer in women is a major public health problem throughout the world.Global breast cancer incidence increased from 641 000 (95% uncertainty intervals 610 000—750 000) cases in 1980 to 1 643 000 (1 421 000—1 782 000) cases in 2010, an annual rate of increase of 3·1%. In the quest for novel preventive and therapeutic cancer agents, natural products have received increasing attention in recent years. Plant-derived components such as coronopilin- a sesquiterpene lactone - hold promise to treat a variety of cancers. On the other hand, Prior studies suggested that nanoparticle drug delivery might improve the therapeutic response to anticancer drugs. Poly (N-isopropylacrylamide-co-methacrylic acid) (PNIPAAm–MAA) is cross-linked polymeric nanoparticle. It is one of nanoparticles utilized in the drug delivery system for cancer therapy. The purpose of this study was to investigate anti-cancer potential ofCoronopilin-loaded NIPAAm-MAA nanoparticle on T47D breast cancer cell line. Materials and methods: We have synthesized polymeric nanoparticle encapsulated formulation of coronopilin utilizing the micellar aggregates of cross-linkedNIPAAm-MAA. Cell survival rate and cytotoxic effect of Coronopilin-loaded NIPAAm-MAA nanoparticles on T47D breast cancer cell line were measuredusing48h MTT assay. Results: Data analysis and evaluation of results showed that coronopilin-loaded NIPAAm-MAA nanoparticles has cytotoxic effect on T47D breast cancer cell line and efficiently inhibit(IC50 = 32μg/ml) growth of breast cancer cell population. Conclusions: This study indicates that coronopilin-loaded NIPAAm-MAA suppresses the growth of human breast cancer cells. The treatment of T47D cells with coronopilin-loaded NIPAAm-MAA results in significant cell growth inhibition. Therefore nanocoronopilin opens up avenues for systemic therapy of breast cancer and the other human cancers

کلیدواژه ها:

Breast cancer ، Coronopilin ، NIPAAm-MAA ، drug delivery