Investigation of antioxidant activity of n,n n′-dipyridoxyl -(o-phenylenediamine) schiff base and its effects on superoxide dismutase gene expression in hep g2 cells

Antioxidants are substances that protect the human body against free radicals and prevent cancer cells.Schiff base are known to have biological activities such as antibacterial, antifungal, antitumor andantioxidant properties. The aim of this study is to evaluate antioxidant activity and the effect of Schiff base on the Superoxide dismutase expression as enzyme with antioxidant activity. DPPH (2,2-diphenyl-1-picrylhydrazyl) chemical is used to measure the antioxidant activity of the substances. dissolving first, the DPPH in 96% ethanol form free radicals, which is most absorbed at 517 nm and becomes purple. If any substance has antioxidant activity, decreasing amount of absorbance and solution turn into yellow. Also, real time q-PCR method was performed in order to determine expression of Superoxidedismutase enzyme. To do this, RNA was isolated from HepG2 cells (treated and untreated) using high pure RNA isolation kit (Roche, Germany) according to the company’s instruction. With the use ofreverse transcriptase, cDNA was synthesized using Oligo (dT), Primer. Then, the Real time PCR was done, usingBio-Rad CFX96 detection system. The changes in Superoxide dismutase gene expression in treated cells with the different concentrations of Schiff base (250, 500, 1000 μg/ml) compared to untreated cells. GAPDH was used as housekeeping gene.antioxidant effects of Schiff base in scavenging DPPH free radicals show that the IC50 values are 31 μg/ml. The Real time PCR result showed that the level expression of SOD in exposed cells compared to untreated cells considerably increased to 2.05, 2.1, 3.06 fold in 250, 500 and 1000 μg/ml concentrations of Schiff base. our findings have shown the antioxidant properties of N,N′-dipyridoxyl(O-phenylenediamine) Schiff base, which could be used as antioxidant agent in therapeutic goals such as cancer therapy

کلیدواژه ها:

Gene and Cancer ، Cell and Cancer ، Cancer Risks ، Drugs and Cancer ، Chemotherapy