Targeting the core regulatory network of stemness in an in vitro aml model

A main goal in regard of AML therapy is to tackle two main obstacles; refractoriness to induction chemotherapy and relapse after remission which may be initiated from a rare population of cells called,Leukemia stem cells (LSCs). Here, we review the current approaches in targeting these cells. LSCs are a rare cell population of cancer stem cells that lead to the rapid clonal expansion of immaturemyeloblasts. Interestingly, these cells possess a number of stem cell properties such as quiescence and survival that are linked to therapy resistance. Therefore, targeting these cells could eradicate AML. In general, there are three main approaches in targeting LSCs: 1) Targeting stem cell related molecular pathways 2) Targeting stem cell specific surface markers 3) Targeting the stem cell microenvironment.In recent studies, it is suggested that applying bioinformatic approaches may have an effective role in identifying precise biomarkers or targets in LSCs. Gene Regulatory Network (GRN) is an advisable toolin choosing specific genes, which may have a high probability in regulating the stemness core in LSCs. GRN is considered as an adequate approach in mapping gene interactions and pinpointing hot spotsalso known as hub genes , that have the most interaction with others. By applying this approach and using a defined set of criteria, we can attain a list of genes and perform further studies on them in wetlab. Bioinformatics and in specific, GRN is considered as a useful tool to detect genes that may play a crucial role in LSC signaling pathways and in detail, survival and quiescence signaling pathways that are linked to therapy resistance

کلیدواژه ها:

Blood Cancer ، Cancer Stem Cells ، Stem Cells and Cancer ، Multidisciplinary Cancer Research ، Targeted Cancer Therapy