Apoptosis-inducing effects of zinc oxide nanoparticle synthesis by the origanum majorana in human breast cancerous cells (mcf-7)

Nanoscience has a great potential in diagnosing and treating various diseases, including cancer. A lot of research has been done on the use of some nanocarriers, such as liposomes, mycelia and nanoparticles, to detect and control of various cancers. The green method and the use of plants in nano synthesis due to the low toxicity and therapeutic properties of plants has been very much consideration. ZnO nanoparticles due to certain physicochemical properties have been acting as a powerful agent in the treatment of cancer. Medications and nanoparticles with specific strategies inhibit cancer cells which induces apoptosis and prevents proliferation of cells, including these methods. In this study, the effect of cytotoxicity and induction of apoptosis in breast cancer cells exposed to ZnO nanoparticles was evaluated. MTT Assay: Initially, the mean concentration (IC50) was determined using the MTT test. For this test, the cells were grown in 96 well plates and, after attaching to the bottom of the plate, were treated with different concentrations of the nanoparticle. Then the MTT solution was added to each well to determine the survival of the cells. Finally, the amount of absorption after addition to DMSO was recorded using ELISA Reader. Fluorescence Microscopic Observation of Cell Death: In this method, acridine orange/propodium iodide (AO/PI) double staining was used. Differences in thecolor of the cells indicate the occurrence of apoptosis in cells. The cells were seeded in 6 well plate and treated with three concentrations including IC50 (25μg/ml), lower (12.5 μg/ml) and higher (50 μg/ml) than the IC50. The cells were stained and examined using a fluorescence microscop. The results of the toxicity test show that the cell survival rate is reduced by increasing the concentration of thenanoparticle and, on the contrary, toxicity increases. In other words, the nanoparticle is capable of inhibiting cancer cells with a IC50 about 25 μg/ml 24 h after treatment. The survival rate decreasedfrom a concentration of 15.6 to 125 μg/ml about 70 %. The viable (green), apoptotic (orange) and necrotic (red) cells were observed using fluorescence microscope. A concentration dependent increasein induction of programmed cell death was observed. As the concentration increased, the number of orange and red cells increased and the green cells diminished. This nanoparticle is capable of inhibitingcancer cells by inducing apoptosis, that these results can introduce this nanoparticle as a suitable candidate for cancer cell suppression

کلیدواژه ها:

Breast Cancer ، Cancer Prevention ، Cell and Cancer ، Cancer Treatment and Management ، Drugs and Cancer