In vitro study of cytotoxic effects of zn o nano particles on cancerous (hepg2) and normal cells (l929)

Nowadays, there are significantly attention in the study of plants as a medicinal agent. Nanotechnology has been provided important treatment for cancer sick persons. Biological Nanoparticles have specific properties such as Biocompatibility , cytotoxicity features and high stability. exposure of normal cell to Nano particle can determine the toxicity of Nanoparticles against these cells that knowing such information can be effective in controlling cancer cell in living tissue .in order to this study is concentrated on Zn O nanoparticles (NPs) and its cytotoxic effects on liver cancer cells (HepG2) andnormal cells (L929). MTT assay is the best test to determine the cytotoxic effects on cells. First we permit the cells reaches the logarithmic phase .in this phase , the cells have the most growth anddivision. Then mice fibroblast L929 cells injection to each well plates contain 100 μl DMEM high glucose medium. After that incubated in humidified atmosphere of 5% CO2, 95% air at 37c. Variousconcentrations of Zn O nanoparticles (NPs) prepared and treated cell. After 24,48 and 72 h of incubation 15 μl of MTT solution was added to every wells. the cells incubated for 4 h at dark location .Then, the formazane crystals that derived from the interactions of the enzyme in the mitochondria of the living and MTT solution, were dissolved in DMSO solution. Finally, the absorbance was recorded at 570 nm. Determination of the toxicity of the compounds and the bioavailability of the exposed cells can be evaluated by the MTT assay. The results of this survey showed the survival cell has an inverse relationship with increasing the concentration of the Zn O nanoparticles (NPs). The results showed that the L929 survival rate of cells at 24, 48 and 72 h after treatment was reduced from 80% to 10% at concentrations of 1.2 and 2.4 μg/ml respectively. Investigating the amount of inhibition on cancer cells showed that about 50% of the cells were inhibited 72 hours after treatment at 0.5μg/ml. Also, significant changes were also observed in the shape and size of treated cells compared to control (untreated cells) on both cell lines reviewed. The result of this study show that low concentration of Zn O Nanoparticles can stop growth of cells , but comparing the amount of inhibition against normal cells compared with cancerous cells 72 hours after treatment showed that the nanoparticle can inhibit HepG2 cancer cells in lower concentrations relative to the normal cell. Such information can be used to prevent damage to normal cells in the treatment of cancer.

کلیدواژه ها:

Cancer Prevention ، Cell and Cancer ، Cancer Risks ، Cancer Treatment and Management ، Drugs and Cancer