Evaluation of toxicity and apoptogenic properties of purslane on glioblastoma multiforme cancer cell line (u- 87): the role of no

Glioma, the tumors caused by glia cells, is the most common type of primary brain tumors, in particular malignant glioma considered as the deadliest type of adult brain tumors. More than 50 percent of all kinds of glioma are dedicated to Glioblastoma. The overall incidence of glioblastoma is 2-3 people in a hundred thousand that is more common in men over sixty years old. It also includes 20 percent of totalintracranial tumors. Despite advances in understanding about glioblastoma pathophysiology and its therapy, it is yet too considered curable. Therefore, new therapeutic methods and new drugs are quitenecessary. In the present study, we aimed to evaluate the cytotoxicity and apoptogenic effects of hydro- ethanolic extract of Portulaca oleacea on human glioblastoma cancer cell line (U-87) and the role ofNO. Cytotoxicity of the extract was measured using MTT assay, and the following hypotonic PI and SubG1 peak were done to have better insight to apoptosis. Moreover, the NO, Urea and NO/Urearatio were evaluated. All tested were performed after 24, 48 and 72 h. The extract had cytotoxicity and apoptogenic effects on U-87 cells with concentration and time dependent manners. The mechanism ofcytotoxicity and apoptosis induction of the extract with time lapse and higher concentrations was with NO, U and NO/U ratio pathway. PO significantly raised NO level and markedly reduced urea levelcompared to control group. In addition, NO/urea ratio was notably augmented by PO.Present study may suggest the cytotoxicity and apoptogenic effects of Portulaca oleracea along with upstream NOmechanism. Furthermore, the extract as ethnobotaniocal may be used as adjunctive anti-cancer activity against glioblastoma multiforme

کلیدواژه ها:

Cell and Cancer ، Solid Tumors ، Cancer Treatment and Management ، Drugs and Cancer ، Chemotherapy