Comparison cytotoxicity of the nanomicelle and free curcumin against human primary glioblastoma cell line

Curcumin, a natural polyphenol derived from the rhizomes of turmeric (Curcuma longa), is a highly lipophilic molecule. Although curcumin showed potent anticancer and cancer preventive effects due totargeting various signaling pathways its clinical usage is limited due to poor bioavailability. Nanomicelle formulations of curcumin have been developed to enhance the bioavailability and the solubility of thislipophilic compound. Turmeric (Curcuma longa Linn), is a crystalline compound which has been traditionally used in medicine and cuisine in India. Curcumin (diferuloylmethane) is the major active component of turmeric. Curcumin has been shown to be cancer chemopreventive in several different animal tumor bioassay systems including colon duodenal, stomach, prostate and breast carcinogenesisboth in-vitro and in-vivo. Absence of dose limiting toxicity, when curcumin is administered up to 8 g/day in human clinical trials, reveals the possibility of curcumin in the prevention and treatment of cancer. Nano-Curcumin is a registered curcumin product (SinaCurcumin®) for use which has been developed in Nanotechnology Research Center of Mashhad University of Medical Sciences, Mashhad, Iran and marketed by Exir Nano Sina Company in Tehran-Iran (IRC:1228225765). Each soft gel of Nano-curcumin contains 80 mg of curcumin in the form of nano-micelle. Human glioblastoma astrocytoma cells (U-87 MG) were derived from a malignant glioma (European Collection of Cell Cultures, Salisbury, UK). In this study the cytotoxicity of curcumin nanomicelle against human primary glioblastoma cell line was evalution in vitro by MTT assay . Size of the particles was measured by particle size analyzer ( Malvern). Our results indicated that the IC50 concentration of the micelle form of curcumin showed lower toxicity than that of non-micelle form (22.577 μM vs 15.854 μM respectively). size of the A nanomicelle particle is 30 nm.

کلیدواژه ها:

Cancer Prevention ، Cell and Cancer ، Nutrition and Cancer ، Cancer Treatment and Management ، Drugs and Cancer