Laccase is one of the best enzymes for biodegradation of aromatic compounds. Anthracene
is a polyaromatic hydrocarbon with high toxicity for environment. Degradation of anthracene has a high priority.A lot of Basidiomycetes can degrade anthracene with producing laccase and other ligninolytic enzymes.In this study laccase was isolated from a local wood decaying fungus. Effect of the enzyme was studiedon biodegradation of anthracene in different temperatures and pH by gas chromatography analysis inpresence and absence of iron oxide nanoparticles. The best temperature for biodegradation in presence ofnanoparticles increased, but optimized pH didn’t change. Biodegradation
of anthracene efficiencies wasobtained 73% in absence to 88% in presence of nanoparticles. Laccase
has improved function by Ironoxide nanoparticles and this function remained more time. This study showed the application of laccasewith iron oxide nanoparticles for improved anthracene pollutant control.