Inhibitory Influence of 3-β-hydroxybutyrate on Calf Thymus DNA Glycation by Glucose

Reactions of reducing sugars with free amino groups of proteins can produce advanced glycation endproducts (AGEs). DNA can react with sugars in a similar way as proteins [1]. AGEs have a significantrole in the pathophysiology of aging and diabetic late complications because of their genotoxic effect[2].The 3-β-hydroxybutyrate (3BHB), a ketone body which is produced by the liver, can be detected inincreased concentrations in individuals post fasting and prolonged exercises and in diabetic (type I)patients[3]. Although several studies have been done on protein glycation, but fewer studies were carriedoutregarding DNA glycation. In this study,to check the inhibitory effect of 3BHB on DNA-AGEsformation, Calf Thymus DNA with glucose in presence and absence 3BHB was incubated on thephysiological-likeconditions for 4 weeks.After dialysis of samples,to monitor the non-enzymaticglycation of DNA nucleosides and to characterize the formation of nucleoside AGEs, the biophysicaltechniques such as circular dichroism (CD), agarose gel electrophoresis, UV–vis and fluorescencespectroscopy were used.The results ofCDshowed,native DNA had a negative peak of -6.1 mdeg at 245nm, and a positive peak of +16.4 mdeg at 275 nm. DNA modified with glucose alone and DNA modifiedwith glucose+3BHB showed -3.4 and -4.5 mdeg for negative peaks at 245 nm, and 10.9 and 13.2 forpositive peaks at 275 nm, respectively.Thus, 3BHB with decrease AGEs formations can decrease thestructure changes of DNA and can prevent from breaking of DNA in diabetic conditions.

کلیدواژه ها:

DNA ، Glycation ، 3-β-hydroxybutyrate ، Ketone body