Production of pectinase from sugar beet pulp byPiriformospora indica under submerged fermentation
محل انتشار: چهاردهمین همایش بیوشیمی فیزیک ایران
سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 695
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شناسه ملی سند علمی:
CBC14_045
تاریخ نمایه سازی: 7 اسفند 1396
چکیده مقاله:
The biotechnological potential of pectinolytic enzymes from microorganisms has drawn a great deal ofattention from various researchers. Pectinases are one of the upcoming enzymes in food processingindustries and they hydrolyze pectin substances. Pectinases production could be induced by agriculturalwastes containing pectin such as, sugar beet pulp, apple pomace and citrus peel by variousmicroorganisms. Sugar beet pulp (SBP) is by- product of sugar factory and especially rich in pectin. Inthis study, submerged fermentation was used for the production of pectinase by Piriformospora indicafungus with sugar beet pulp powder [1-3]. For further analysis the effect of supplementation of mediumwith glucose (0-10%) and ammonium sulfate (0.1-0.5 %) were evaluated. At the 6th day of culture, thecell growth, growth yield, specific growth rate, spore yield, total protein content and pectinase activitywere estimated. According to the results, maximum fungal biomass production, protein content andenzyme activity were determined at glucose 6% and ammonium sulfate 0.4%. Moreover, the highestpectinase activity at glucose 6% and ammonium sulfate 0.4% was calculated 4.82 U/ml and 4.29 U/mlrespectively. All together, SBP could be a good inducer for pectinase production by P. indica and furtherinvestigation is running.
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نویسندگان
Parisa Fathi Rezaei
Biology department, Faculty of Science, University of Maragheh, Maragheh, Iran
Somayyeh Kiani
Biology department, Faculty of Science, University of Maragheh, Maragheh, Iran
Saleh Shahabivand
Biology department, Faculty of Science, University of Maragheh, Maragheh, Iran