Production of pectinase from sugar beet pulp byPiriformospora indica under submerged fermentation

The biotechnological potential of pectinolytic enzymes from microorganisms has drawn a great deal ofattention from various researchers. Pectinases are one of the upcoming enzymes in food processingindustries and they hydrolyze pectin substances. Pectinases production could be induced by agriculturalwastes containing pectin such as, sugar beet pulp, apple pomace and citrus peel by variousmicroorganisms. Sugar beet pulp (SBP) is by- product of sugar factory and especially rich in pectin. Inthis study, submerged fermentation was used for the production of pectinase by Piriformospora indicafungus with sugar beet pulp powder [1-3]. For further analysis the effect of supplementation of mediumwith glucose (0-10%) and ammonium sulfate (0.1-0.5 %) were evaluated. At the 6th day of culture, thecell growth, growth yield, specific growth rate, spore yield, total protein content and pectinase activitywere estimated. According to the results, maximum fungal biomass production, protein content andenzyme activity were determined at glucose 6% and ammonium sulfate 0.4%. Moreover, the highestpectinase activity at glucose 6% and ammonium sulfate 0.4% was calculated 4.82 U/ml and 4.29 U/mlrespectively. All together, SBP could be a good inducer for pectinase production by P. indica and furtherinvestigation is running.

کلیدواژه ها:

submerged fermentation ، sugar beet pulp ، Piriformospora indica ، pectinase ، pectin