Study of the simultaneous effect of silver nanoparticles and 3-β-hydroxybutyrate on human hemoglobin glycation process

Hemoglobin is the first protein in the glycation studies.Glycation is sugar binds to macromolecules whichcause complications of diabetes [1] such as cataract and hardening of the arteries. Several factors areeffective in the protein glycationsuch as pH, temperature, concentration, chemical reagents [2]. In thisstudy,the effect of simultaneous silver nanoparticles and 3-β-hydroxybutyratewas studied on humanhemoglobin glycation process on the physiological-like conditions. All samples were dialyzed after 42days of incubation and were characterized using various biophysical techniques such as UV–vis, circulardichroism and fluorescencespectroscopy. The results offluorescence in excitation 320 nm, showed thatfluorescence intensity of hemoglobin samplesincubatedwith glucose alone showed the highest intensityamong all samples. The fluorescence intensity was reduced about24% in the present of 3BHB with silvernanoparticles.The simultaneous presence of silver nanoparticles and 3-β-hydroxybutyrate could help toreduce the effects of glucose. Therefore, silver nanoparticles can reduce the glycation process which iseffective in reducing complications of diabetes.

کلیدواژه ها:

Glycation ، human hemoglobin ، 3-β-Hydroxy butyrate ، Silver nanoparticles