A Capacitance based label free aptasensor for botulinum neurotoxin detection

As the botulinum neurotoxins- spatially type A- have more importance for both clinically and industriallyarea, treeing to finding fast and fasil method to determination of this toxin is favourite for scientists andpoliticiansHere we reported afasil approach to achieving anano biosensor for botulinum neurotoxin typeA(Bont/A). Usage a Nano electrode based on modified graphene-carbon pasteand by manse LCR meterwe investigate the present of toxin in buffer media. At first graphene nanocomposites was synthesischemically,while itshows a good conductivity witch it wassensitive for determination of Bont/A.Immobilization of aptamer has been done on the Graphene electrode in 100mM phosphate buffer solution(pH = 7) randomly. The Bont/A was cloned and expressed in E.coli, then toxin was harvested andpurified and confirmed using the genetic and biotechnological methods.Graphene modified electrodeswere applied as indicator for capacitance determination of Bont/A. In this study, SEM, electrochemicalimpedance spectroscopy and cyclic voltammetry techniques have been used to confirming the wellfabrication of the nanobiosystem. The target toxin was detected in a range from 1.5×10 -1to 6.5×10 -1ng/mL.Through this study, BoNT/A was detected with detection limit of 0.09 ng·mL-1and correlationcoefficient was about 0.964.

کلیدواژه ها:

Graphene ، aptamer ، Botulinum ، biosensor ، electrochemistry