Protocol of optimization tomato tissue culture for the micropropagation and recombinant protein production (Feron genotype)

For years, plants used for the production of recombinant proteins and as Molecular farming is known. However, the transformation of tomatoes is still not A common method and repeatable for all digits. In this study, we’ve optimized the tissue culture of tomato genotype (Feron genotype) with two cotyledon and hypocotyl explants in 6 different culture medium whit different hormones. In the meantime, culture medium supplemented whit benzyl amino purine (BAP) mg/L3 and Zeatin mg/L1 In addition whit 0.5 mg/L indole acetic acid (IAA) showed the highest regeneration rate. Interaction among Culture medium and explants on callus induction and regeneration were observed. Among the explants cotyledon was the best regeneration.

کلیدواژه ها:

tomatoes ، optimize tissue culture ، the role of hormones