Effects of the Incubation Time in Immobilization of Protein on Styrenic copolymer

Enzymes, the catalysts of living organisms, have been used in solution as homogenous catalysts, but for many applications this is not the most efficient way to use them. Today, enzymesare usually immobilized on a solid support (1-6).The ideal support for a given application is one which would increasesubstrate binding, decrease product inhibition, shift the apparent pH optimum to the desired value, increase enzyme stability, discourage microbial growth and be readily recovered for reuse. In the present research, copolymer of styrene-maleic anhydride was used as a support for trypsin immobilization. Important parameters were studied