Immobilization of Cellulase on Modified Chitosan Beads for increase of Stability

In this invention we have investigated immobilization of cellulase onto chitosan polymeric beads and characteristics of immobilized enzyme have been studied. Chitosan beads were coated with polyvinyl alcohol improving by maleic anhydride and cross-linked by Epichlorohydrin. As a result of this modification is shown, optimum pH for cellulase activity shifted from 4 to 8 for immobilized enzyme, while the optimum temperature for both immobilized and free enzymes were found to be nearly the same. The optimum enzyme activity of the immobilized cellulase was found to be 91.5% of free cellulase enzyme. It is clear that immobilized cellulase beads are more stable and better suited for industrial applications.

کلیدواژه ها:

Cellulase ، Immobilized enzyme ، Polyvinyl alcohol ، Epichlorohydrin ، Chitosan