Plant regeneration via somatic embryogenesis and organogenesis in Verbascum speciosum Schard
محل انتشار: مجله سلول و تحقیقات مولکولی، دوره: 4، شماره: 2
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: فارسی
مشاهده: 436
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شناسه ملی سند علمی:
JR_JCMR-4-2_005
تاریخ نمایه سازی: 7 بهمن 1395
چکیده مقاله:
Plant regeneration was achieved in Verbascum speciosum Schard. via organogenesis and somatic embryogenesis by culture of mature embryo explants. Two types of calli, embryogenic and non-embryogenic,were induced from mature embryo explants on Murashige and Skoog (MS) medium supplemented withdifferent concentrations of benzyl adenine (BA) and α-naphthalene acetic acid (NAA). In order to further proliferate the somatic embryoids, the yellow and friable embryogenic calli were transferred on MS medium containing 0.5 mg-1 charchol and 0.1 or 1 mg-1 2,4-dichlorophenoxy acetic acid (2,4-D) or into MS mediumcontaining 60 g-1 sucrose, 50 mgl-1 casein hydrolysate (CH), 0.5 mg-1 kinetin (Kin), 5 mg-1 2,4-D and 0.5 mg-1charchol. Among the 3 tested media, MS medium containing 0.1 mg-1 2,4-D and 0.5 mg-1 charchol was more effective for proliferation of embryonic calli. Somatic embryos were transferred to hormone free MS medium for maturation and shoot regeneration. In addition, shoots and roots regenerated from non-embryogenic calli inhormone free MS medium or containing NAA and BA. Shoot buds were obtained from non-embryogenic calli and they were transferred to MS medium supplemented with 1 mg-1 BA or Kin for further growth and multiplication. Regenerated plants then were potted and maintained in the greenhouse
کلیدواژه ها:
نویسندگان
roya karamian
Laboratory of Plant Physiology, Department of Biology, Faculty of Science, Bu-Ali Sina University, P. O. Box 65175/4161,Hamedan, Iran
fatemeh ghasemlou
Laboratory of Plant Physiology, Department of Biology, Faculty of Science, Bu-Ali Sina University, P. O. Box 65175/4161,Hamedan, Iran