Improvement of Thermal Stability of DFPase by In silico Methods
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 465
فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JABR-1-4_004
تاریخ نمایه سازی: 29 آذر 1395
چکیده مقاله:
Efficiency of enzymes which are used in industrial or environmental applications is highly dependent on their thermal stability. In this study, the stability of DFPasehas been evaluated after introducing disulfide bonds to the structure. The results obtained from a series of protein design software were subjected to moleculardynamics simulation at different temperature to test the performance of such combinatorial procedure. Amount several designs, mutation M5 showed desirablethermostability via molecular dynamics simulation and normal mode analysis. As it clearly depicted, such in silico structural investigations would be resulted in reducing the numerous choices of experimental options as it was undergone a series of computational evaluation previously.
کلیدواژه ها:
نویسندگان
Morteza Mirzaei
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Mohammad Latifi
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Rahim Jafari
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran