Optimization of Callus Induction in Pennyroyal (Mentha pulegium)

سال انتشار: 1393
محل انتشار: فصلنامه گزارش های زیست فناوری کاربردی، دوره: 1، شماره: 3
کد COI اختصاصی: JR_JABR-1-3_002
زبان مقاله: انگلیسی
تعداد مشاهده: 630

نویسندگان

Agronomy and Plant Breeding Department, Razi University, Kermanshah, Iran

Science and Research Branch, Islamic Azad University, Kurdistan, Iran . Social Security Organization, Kermanshah, Iran

Agronomy and Plant Breeding Department, Razi University, Kermanshah, Iran . Biotechnology for Drought Resistance Research Department, Razi University, Kermanshah, Iran

Sohbat Bahraminejad

Biotechnology for Drought Resistance Research Department, Razi University, Kermanshah, Iran Science and Research Branch, Islamic AzadUniversity, Kurdistan, Iran

چکیده

Findings in relation to the inhibitory effects of natural substances against microorganisms showed that herbal products may be alternatives to synthetic and chemicaldrugs due to their significant therapeutic effects. The pennyroyal (Mentha pulegium) as an important medicinal plant, belongs to the Lamiaceae family is applied inthe pharmaceutical and food industries. In recent years, plant tissue culture techniques have appeared as a powerful tool for the micropropagation and breeding of many plant species. The aim of current study was to find the best medium compositionfor callus induction in M. pulegium. For this purpose, three explants (leaf, root and stem) of M. pulegium were cultured on MS medium supplemented with BAP(0.5 and 1.0 mg/L) and 2,4-D (0.0, 1.0, 2.0 and 4.0 mg/L) in a factorial experiment. The results showed that the leaf explant had the highest effect on callus induction(9.65 mm in callus diameters at 28 days after culture) and hormone levels of 1 mg/L 2,4-D and BAP-free identified as the most efficient concentrations for callus growth rate (0.29 mm/d). Also statistical analysis demonstrated that among interaction effects t he leaf explant in 1 mg/l 2,4-D and BAP-free found as the highest effect for callus induction (12.35 mm in callus diameter at 28 days after culture).

کلیدواژه ها

Mentha pulegium, Callus Induction, Medicinal Plants, Explant, Hormone

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