Inhibitory Effects of Peripheral Blood Mononuclear Cells Against Myelogenous Leukemia Cell line After TreatmentBy Doxorubicin

Peripheral Blood Mononuclear Cells (PBMC), contain Natural killer (NK) cells that arelymphocytes distinct from B and T cells. Their killing function without a need for clonalexpansion and differentiation, which is required for effector responses of the immune systemsother killer cells. Myelogenous leukemia cells are a good target for NK cells and The cellstolerate some of the chemotherapy regimens in leukemia and maintain their killing function.Non-adherent PBMC to number of 〖2×10〗^(6 )cells/ml in Vitro condition weretreated by 0.4 Âμg/ml 〖ADRIAMCIN〗^® CS is produced by Pfizer, contains 2 mg/mldoxorubicin hydrochloride into each well of 24-well plate for 24 hours. After incubation timecells were washed twice and 100 Âμl of cells suspension, 100 Âμl from 〖2×10〗^5/ml untreated K562 cells and 100 Âμl cell culture media, co-cultured in each well of 96-wellplate for 24 hours in Effector:Target (E:T) ratio of 10:1. Control samples from untreatedeffector and target cells alone and E:T co-cultured cells as control, incubated at the sametime. In order to measure non-adherent PBMC doxorubicin 〖IC〗_50values weincubated the same number of effector cells in 24-well plate with various concentration of thedrug (0.5 †1 Âμg/ml) for 48 hour. All cells viability was measured by Methyl-Thiazol-Tetrazolium (MTT) assay. Non-adherent PBMCs were stimulated by 40 Âμg/mlphytohemagglutinin. Optimal Density (OD) of untreated E:T co-cultured cells as control were1.318 ± .022 (Mean ± SD). OD of treated E and untreated T co-cultured cells were 0.338± .019. 3; Total amounts of E and T cells OD alone were 0.443 ± .028. Statistical analysisusing Paired Samples T Test displayed significantly differences 1 and 2 with 3 (p<.01) but 1and 2 had no significant differences (p>.05). PBMC doxorubicin 〖IC〗_50values was0.823 ± .017 Âμg/ml (Mean ± SE). Thus treated effector cells maintain their killing function.

کلیدواژه ها:

Doxorubicin ، Inhibitory Effects ، Killing Function ، Effector Cells ، Target Cells