Strong Linkage Disequilibrium Between -670 A>G and - 1377 G>A Polymorphisms in FAS Gene in Patients withBreast Cancer and C Ontrols

Breast Cancer is the second cause of mortality in women. the etiology of breast cancer ismulti factorial. genetic factors are participated in the etiology of breast cancer. The FAS gene,has a critical role in the tumor growth and metastasis. Gene polymorphisms including -1377G>A and -670 A/G in FAS genehave shown to change the transcription activities of this gene.The FAS genotypes were determined by polymerase chain reaction-restriction fragmentlength polymorphism (PCR-RFLP) in 115 breast cancer patients, 115 healthy controls, allfemale and selected from city of Mashhad in northeastern Iran.BstuI and ScrfI were recruitedas endonuclease enzymes to detect -1377G/A and -670A/G gene polymorphisms,respectively. GG, GA and AA genotype frequency for FAS -1377 polymorphism in patientswas 30.4%, 49.6% and 20%, whereas in controls was 26.1%, 50.4% and 23.5%, respectivelyand there was no significant difference between case and controls (p=0.7).Additionally,genotype frequency of FAS -670 AA, AG and GG was 52.2%, 39.1% and 8.7%respectively in patients. Result of genotype frequency in controls for FAS -670 AA, AG andGG was 47%, 41% and 10.4% respectively, which Showed insignificant difference ascompared with patients genotypes (p=0.78). These results show that FAS−1377G/A andFAS -670 A/G gene polymorphisms have a strong linkage disequilibrium with p value <0.001.These results indicate the lack of association between FAS−1377G>A and FAS -670 A/Ggene polymorphismsand risk of breast cancer. Additionally, a significant linkagedisequilibrium is found between FAS−1377G/A and FAS -670 A/G gene polymorphisms incase and control groups.

کلیدواژه ها:

Breast Cancer ، -FAS−1377G>A ، FAS -670 A/G ، Linkage Disequilibrium ، GenePolymorphism