Evaluation of the Effects of 0.25 and 2.5 mT Extremery Low Frequency Electromagnetic Field on CDH1 GeneExpression in  Colonic Carcinoma (HCT-116) Human Cell Line

Previous studies have shown that cells and tissues can be influenced by electromagneticfields exposure. Electromagnetic fields have physiological effects on cells and tissue such asalteration of gene expression , cell viability, proliferation , apoptosis and cell cycle phase.Impact of electromagnetic fields depends on density , frequency range and exposure time.Epithelial-mesenchymal transition is the process by which epithelial cells lose cell-celljunctions and baso-apical polarity and acquire plasticity, mobility, invasive capacity, stem-likecharacteristics, and resistance to apoptosis. Epithelial mesenchymal transition is one ofimportant biological process in embryonic period, wound healing and metastasis. E-cadherin(CDH1 gene) downexpression has been associated with Metastasis.it is well-known as atumor suppressor gene. Evaluation of the effects of effect of electromagnetic field on Ecadherinexpression. HCT-116 cell line cultured and exposed in 4 group : 1) 0.25 mT in 12hour alternative 2) 0.25 mT in 12 hour continuing 3) 2/5 mT 12 hour alternative 4) 2/5 mT 12hour continuing and CDH1 gene expression compare with control group(without exposure)by real time PCR .exposed groups viability compare with control group by Trypan bluemethod. CDH1 expression increased in 4 group exposed. over-expressions of E-cadherinrelationship with level of Electromagnetic fields and expoture time. In viability analysis,Electromagnetic fields didn’t have effect on cells viability. Electromagnetic fields can beby over-expression of E-cadherin resist Epithelial mesenchymal transition therewith can beresist metastasis.

کلیدواژه ها:

Electromagnetic Field ، Epithelial Mesenchymal Transition ، Metastasis ، Ecadherin ، Cancer ، Â Colonic Carcinoma (HCT-116)