In Silico Analysis Of Interferon gene Cloning In E.coli With Getway Cloning APPROACH

The GATEWAYTM conversion technology (Invitrogen) is based on the site-specific recombination reaction mediated by phage λ.DNA fragments flanked by recombination sites (att) can be transferred into vectors that contain compatible recombination sites (attB × attP or attL × attR) in a reaction mediated by the GATEWAYTM BP ClonaseTM or LR ClonaseTMEnzyme Mix (Invitrogen). The entry clones, which can be considered general donor plasmids, are made by recombining the DNA fragment of interest with the flanking attB sites into the attP site pDONR mediated by the GATEWAYTM BP ClonaseTM . The fragment in the entry clone can be transferred to any destination vector that contains the attR sites by mixing both plasmids and by using the GATEWAYTMLR ClonaseTM Enzyme Mix. Interferons (IFNs) are widely used as human therapeutics.A new class of hybrid IFNs is being investigated for possible human use against cancer and viral pathogens. In this paper described a kind of efficient cloning for therapeutic gen