QUANTITATIVE ASSAY OF SYNTHETIC PEPTIDE GNRH-a BY REVERSE–PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN THE ABSENCE OF A PURE REFERENCE SAMPLE

A reversed-phase high Performance liquid Chromatography method for the quantitative assay of synthetic peptide (Gonadotropin releasing hormone analogue, GnRH-a), is presented. It is based on calculated wxtinction coefficient and area under peak by utilizing inexpensive and eassily available tryptophan instead of certified refrence substance (CRS). Reliability of this method was established by comparison with European pharmacopoeia assay METHOD.

کلیدواژه ها:

Peptide ، GnRH-a ، Extinction coefficient ، Tryptophan ، Reverse-phase HPLC