Optimization of the manufacturing process for Bovine Serum Albumin (BSA) nanoparticles

Nanoparticles prepared by desolvation and subsequent crosslinking of Bovine Serum Albumin (BSA) represent promising carriers for colloidal drug delivery systems. The produced nanoparticles were characterized with respect to size. The particles size is a crucial parameter, in particular for in vivo behavior of nanoparticles after injection. The present study aims at optimizing of the two parameters which can affect on the size of particles. Optimization of two parameters, protein concentration and rate of Ethanol addition was done by full factorial design. The screening presented that concentration has main effect on the mean size. Optimization of protein nanoparticles in the range of 5- 30 mg/ml of BSA was resulted 15 mg/ml has minimum mean size. AFM indicated the semispherical shape of particles .

کلیدواژه ها:

Protein nanoparticles ، Bovine Serum Albumin (BSA) ، particle size distribution ، drug delivery