production of uricase by a new source Mucor hiemalis
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
سال انتشار: 1384
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 2,254
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شناسه ملی سند علمی:
NBCI04_286
تاریخ نمایه سازی: 30 دی 1386
چکیده مقاله:
165 Strains of microorganisms with ability to degrade uric were isolated from soil samples during a screeming program. Among them, a zygomucete fungus with well developed columellae showed to produce high levels of the enzyme in a short time. Isolated fungus was identified as Mucor hiemalis according to the morphological and culture characteristics of the organism. The fungus was able to produce an intracellular urate oxidase in a fermentation medium mainly containing uric acid. Optimized composition of the medium consisted of (1 of distilled water) uric acid, 7.0g, maltose, 6.0 g , Vogel stock solution, 20.0 ml and 1.0 ml of 0.5 M copper sulphate.The optimum pH and temperature for uricase production in the optimized medium were pH 6.0 and 30 C, respectively
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نویسندگان
Mojtaba Tabatabaei Yazdi
Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences
Gholamreza Zarrini
Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences
Elham Mohit
Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences
Mohammad Ali Faramarzi
Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences