Construction and expression of hybrid CS3::his6 pili as a novel biosorbent

Display of peptide sequences on the surface of bacteria is a technology that offers exciting applications in biotechnology. An interesting application area is the display of metal-binding peptide on the surface of bacteria for sequestration of toxic metals in water and waste water.CS3 pili is surface organelle of Enterotoxigenic E.coli and suitable system for display of heterologous peptide. In this study a novel cell surface display system for metal uptake was developed using CS3 pili. Hexa histidin peptide was displayed on the surface of E.coli, This peptide was used for the reason that it is good chelater for divalent metal ions. The recombinant bacteria could find use in uptake of heavy metals from environment.

کلیدواژه ها:

bacterial display ، CS3pili ، biosorbent. Hexa histidine