Integrated expanded bed chromatography for Macromolecular and Nanoparticle purification :column diameter effect on mixing
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
سال انتشار: 1384
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 1,933
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شناسه ملی سند علمی:
NBCI04_219
تاریخ نمایه سازی: 30 دی 1386
چکیده مقاله:
Expanded-bed adsorption (EBA) is a novel technique for primary recovery of proteins and nanoparticle bioproducts starting from unclarified broths. This process is an integration process which combines centrifugation, concentration, filtration, and initial capturing of the bioproduct in a single step. Here streamline adsorbent was loaded into 1 cm column and subjected to hydrodynamic comparison with 1.6 as well as 2.5 cm fluidized bed contactors.the hydrodynamic characteristics (N, D a x i and B º )of 1 cm column demonstrated a minimal mixing which is desirable for Macromolecular (e.g ,enzyme,protein)as well as Nanoparticle bioproduct (e,g ,plasmid DNA ,virus)purification. This contrasted with that of 2.5 cm column , which deteriorated within the same range of study. The generic effect of column diameter on liquid mixing and subsequently bioseparation performance in such integrated processes is discussed .
کلیدواژه ها:
Expanded bed adsorption .fluidized bed ، Integration Processes ، bioproduct purification ، fluid mixing
نویسندگان
M Khavarpour
Nanobiotechnology Lab., facalty of Chemical Engineering , Mazandaran University , Babol
A Abedijaberi
Biotechnology Lab., department of Biotechnology, Faculty of Science, University of Tehran
M Jahanshahi
Nanobiotechnology Lab., facalty of Chemical Engineering , Mazandaran University , Babol
E Vasheghani-F
Department of Chemical Engineering,facalty of engineering., Tarbiat Modarres University, Tehran