Influence of Lanthanide ions on the structural changes of human serum albumin

سال انتشار: 1393
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 620

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شناسه ملی سند علمی:

CBGCONF02_048

تاریخ نمایه سازی: 9 فروردین 1395

چکیده مقاله:

lanthanide ions(Ce3+,La3+,Er3+) induced Conformational changes of human serum albumin, HSA, in phosphate buffer 10mM at pH 7.4 are investigated using isothermal titration calorimetry, ITC, UV and fluorescence emission spectroscopic methods. The results indicate that Lanthanide ions induces irreversible denaturation of the HSA structure. These conformational alterations are accompanied by a significant alteration of secondary structures. The UV absorption intensity of theses complexes decreased and a slight blue shift was observed in the absorbance wavelength with increasing ligands concentration. The fluorescence intensity of HSA increased regularly and a slight red shift was observed for the emission wavelength with increasing ligands concentration, indicating that Lanthanides complex could quench the fluorescence of HSA and changes the microenvironment of tryptophan residue. The increasing of emission intensity of HSA indicates loss of the tertiary structure of HSA. The results obtained from ITC data are in satisfactory agreement with the spectroscopic methods. The strong negativecooperativity of ligand binding with HSA recovered from the extended solvation model, indicates that HSA denatured as a result of its interaction with Lanthanide ions.

نویسندگان

M Shalbafan

Chemistry Department, Imam Khomeini International University, Qazvin, Iran

G Rezaei Behbehani

Chemistry Department, Imam Khomeini International University, Qazvin, Iran

N Gheibi

Department of Physiology and Medical Physics, Qazvin University of Medical Scienc

D Illghari

Department of Physiology and Medical Physics, Qazvin University of Medical Scienc

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