Biological Properties of Streptomyces plicatus, a Potent Donor of Chitinase Gene for Development of Transgenic Plants Resistant to Fungal Pathogens

سال انتشار: 1384
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 2,206

متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

این مقاله در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

NBCI04_057

تاریخ نمایه سازی: 30 دی 1386

چکیده مقاله:

Soil Actinomycetes, particularly (Streptomyces spp.), are valuable tools in biotechnology by providing special genes in development of transgenic plants resistant to phytopathogens. Actinomycetes microflora of the Iranian soils has not been very well explored in searching for biofungicides or use of their antifungal genes for genetic engineering of crops. In our survey, among 156 soil inhabitant Actinomycete isolates, strain 101 revealed strong antagonistic and chitinolytic activities. Antifungal activity of this strain against (Alternaria solani, Alternaria alternata, Fusarium solani) and four phytopathogenic isolates (Pistachio, Cotton, Olive and Potato) of (Verticillium dahliae) was assayed. Strain 101 was able to growth on chitin minimal medium agar and utilized chitin as sole source of carbon and nitrogen and was physiologically identified as a new strain of (Streptomyces plicatus) from Kerman, Iran. It was propagated in fed-batch-cultures and active crude was prepared upon which some biological characterizations performed. The active metabolite (s) was polar, soluble in H2O and methanol but insoluble in non polar solvents. Antifungal activity composed of two types, mycelial inhibition, inhibition of microsclerotia and melanin biosynthesis. Thermal inactivation points of active phases were 70°C and 90°C. Antifungal (chitinase) gene from (S. plicatus) was evaluated a useful candidate for development of recombinant DNAs in genetic engineering of agriculturally important crop plants for increased tolerance against phytopathogenic fungi.

نویسندگان

S Aghighi

Department of Plant Pathology, College of Agricultural Sciences, Bahonar University of Kerman.

G.H Shahidi Bonjar

Department of Plant Pathology, College of Agricultural Sciences, Bahonar University of Kerman.

P Rashid Farrokhi

Department of Agronomy and Plant Breeding, College of Agricultural Sciences, Bahonar University of Kerman.

A Ayatollahi Moosavi

Department of Mycology and Parasitology, College of Medicine, Medical University of Kerman, Kerman.

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Aghighi, S., 2004. Investigation of antagonistic activity of some soil ...
  • Dong, P., Q. Ji, T. Long and G. Ahmat, 1998. ...
  • El-Tarabily, K.A., G.E.St.J. Hardy, K. S iva sithamparam, A.M. Hossein ...
  • Hsu, S.C. and J.L. Lockwood, 1975. Powered chitin agar as ...
  • Kieser, T., M.J. Bibb, M.J. Buttner, K.F. Chater and D.A. ...
  • Kong, L.D., D.D. Tzeng and C.H. Yang, 2001. Generation of ...
  • Kuster, E. and S.T. Williams, 1964. Selection of media for ...
  • Marten, P., S. Bruckner, A. Minkwitz, P. Luth and G. ...
  • Pegg, G.F. and B.L. Brady, 2002. 'Verticillium wilts'. Cromwell Press: ...
  • Shahidi Bonjar, G.H. and S. Aghighi, 2005. Chitinolytic and Micro ...
  • Shirling, E.B. and D. Gottlieb, 1966. Methods for c h ...
  • Snowdon, A.L., 1991. A color atlas of post-harvest diseases and ...
  • نمایش کامل مراجع