SiO2 nanoparticles modified carbon paste electrode as a biosensor for determination of imotif DNA / Tamoxifen interaction

Cytosine-rich DNA sequences can form a highly ordered structure known as i-motif in slightly acidicsolutions. The stability of the folded i-motif structure is a good strategy to inhibit the telomerase reaction in cancer cells.Tamoxifen (Tam) is an antiestrogen hormonal agent for treatment of breast cancer for more than two decades. In thecurrent study, we designed an electrochemical biosensor by modifying a carbon paste electrode (CPE) with Sio2nanoparticle to investigate ligands which are able to interact with i-motif structure. The Tam drug was used as the modelligand and its ability for stabilizing i-motif structure was examined. The interaction between i-motif DNA and the drugTamoxifen (Tam), was studied in PBS buffer and [Fe (CN) 6]3- through the cyclic voltammetry (CV) and square wavevoltammetry (SWV) methods.

کلیدواژه ها:

i-motif DNA ، Tamoxifen ، Anti-cancer ، Electrochemical biosensor