Identification of combination forming the essence and anti-microbe of alcoholic and hydro-alcoholic extract of Euphorbia helioscopia plant of Qom region

Since, some of herbal extracts including Euphorbia helioscopia have anti-bacterial effects , they can be used as anti-microbial factors in treating infections [1, 2]. The assessment of the essence combination and the anti-microbial quality of stem’s extract of this plant is the purpose of this study.In this empirical study, the stem of Euphorbia helioscopia plant was gathered from the Tinoj village of Qom in the end of the spring 1392. This plant’s essence was extracted by the distillation method and the combination of this essence was separated and identified by the chromatography /mass spectrometer GC/MS and the column DB-5, and extract-making on the Euphorbia helioscopia plant was performed by different solvents and use of Soxhlet system and the soaking method. Extract –making was performed by solvents such as methanol, ethanol, hexane, distilled water and water-ethanol. Anti-microbial activity of this plant identified by measuring the diameter of controlling haloand the minimal density of controller of the growth (MIC) for levels: Escherichia coli ،Staphylococcus aureus؛ Pseudomonas aeruginosa, Salmonellatyphi, Candida albicans and by method of Plait sump and diluting in cultivate liquid environment, respectively. Finding showed that the extracts of stem showed more effect on Pseudomonas aeruginosa Bactria that there was a significant difference with standard antibiotics. According to performed study, it can be concluded that extracts of the stem of Euphorbia helioscopia plant has the high anti-bacterial activity.