Investigation of association among SNPrs:12676 in CHDH gene and azoospermia in Iranian population

سال انتشار: 1393
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 806

متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

CIGS13_0104

تاریخ نمایه سازی: 7 بهمن 1393

چکیده مقاله:

Introduction: Infertility is a complex clinical problem that affect of couples. Half of these cases arise from male factors. Azoospermiais a condition in there is no sperm in semen of patient.CHDH codes the Choline dehydrogenase enzyme which acts in mitochondria and produces ATP moleculeand has a role in it,s motility. Purpose: A case-control population study was performed to:1) find the diversity among the two different populations of Iran, the North and the Center in totally 58 samples, 2) investigation the association of the SNPrs: 12676(G T) in CHDH with azoospermia in both groups. Material and methods: Two separated population of Center and North of Iran( In Royan Institute)were collected .A 243segment of the gene was amplified using PCR.all sampleswere genotyped using direct sequencing.Statistical analyses such as: Chi-squared test and Odds Ratio to investigate the association of the SNP and azoospermia, Phylogenetic trees including: Neighborhood Joining, UPGMA, Maximum Parsimony and Maximum Likelihood trees toinvestigategenetic differences among the population, AMOVA to investigate the genetic difference in populations, K-Means and STRUCTURE analysis toinvestigate of continuity versus stratification, PCOA analysis to find the relation between individuals in each population. The network in which CHDH acts was found in SWISS-Prot data base. Result: there was no significant difference among our populations which meant they were almost genetically similar. We alsofind a positive association between the SNP and azoospermiainfertility in both of underlying populations

نویسندگان

Narjes Bakhtari

Tehran, ShahidBeheshti University

Masoud Sheidai

Tehran, ShahidBeheshti University

Hamid Gurabi

Tehran, Royan Institute

Esmat Ghalkhani

Tehran, ShahidBeheshti University