Evaluation of aqeouus and methanolic extract green tea and losing weight in obesity mice

Green tea consumption has been recommended to have defensive activity against many chronic diseases, including Metabolic Syndrome such as type 2 diabetes It has been observed in mice that green tea reduce blood glucose levels and Indeed losing weight when included in the diet. Green tea polyphenols may influence regulation of blood glucose levels at a number of diverse points including at the level of carbohydrate digesting enzymes, specifically by inhibiting enzymes α- amylase and α-glucosidase . Two special types of α-amylase exist in the human body, salivary α-amylase and pancreatic α- amylase. Both types of α-amylase aid in the digestion of starch. Methods: NMRI female mice were randomly divided into two groups. Test group and control group took high fat diet food (including sesame seeds and creamy biscuits) during 8 weeks and only test group took aqueous and methanolic extract green tea during the same time. After 8 weeks, body weight and biochemical parameters of the two groups (alpha amylase) were measured. Liver hepatocytes were isolated using enzymatic degradation method. After RNA isolation and cDNA synthesis, real time PCR was done with specific primer for the Amy-1 gene and Then, the data were analyzed by SPSSsoftware. Results: We found that aqueous and methanolic extract green tea dose-dependently inhibited α-amylase in a cell-free system and hascrucial role in losing weight Correspondingly ,results showed significant association between aqueous and methanolic extract green tea and losing weight in obese mice. Conclusion: In the present study, we examined the acute effect of aqueous and methanolic extract green tea on the digestion and postprandial effects of oral carbohydrates in mice. We report that cotreatment with aqueous and methanolic extract green tea at a dose equivalent to 1 cups of green tea reduced the postprandial elevation in blood glucose levels and induced losing weight in obese mice .

کلیدواژه ها:

α-amylase ، aqueous and methanolic extract green tea ، obese mice ، losing weight