Curcumin Antibacterial Effects on Mycoplasma hominis clinical isolates from Vaginosis

Background: Mycoplasma hominis is involved in the development of non-gonococcal urethritis, bacterial vaginosis, postpartum fever, and rarely bacteremia, peritonitis, arthritis, and meningitis. This study investigated the effect of curcumin against M. hominis species isolated from vaginosis. Materials & Methods: About ۵۵۰ samples from female patients with vaginosis were examined. The minimum inhibitory concentration (MIC) ranges of tetracycline, doxycycline, clarithromycin, azithromycin, moxifloxacin, ciprofloxacin, and erythromycin were determined. For this purpose, dilutions ranging from ۰.۲۵-۴ µg/mL were prepared. In addition, curcumin compound was diluted at ۵ and ۱۰ µg/mL concentrations and added to the bacterial suspension in SP-۴ broth medium. qRT-PCR was performed to examine the MH۱۶srRNA gene expression upon exposure to ۱۰ µg/mL curcumin. Findings: Of the ۵۵۰ samples, ۲۰ were positive for M. hominis. The patients’ age ranged from ۲۳-۳۵ (mean: ۲۶.۵±۲) years. The MIC ranges of antibiotics included ۱->۴ µg/mL for doxycycline, ۲->۴ µg/mL for clarithromycin, ۲->۴ µg/mL for azithromycin, ۲->۴ µg/mL for moxifloxacin, ۲->۴ µg/mL for ciprofloxacin, and ۱->۴ µg/mL for erythromycin. Exposure to ۵ µg/mL curcumin resulted in the inhibition of eight isolates. Curcumin at a ۱۰ µg/mL concentration inhibited the growth of ۱۵ out of ۲۰ isolates after ۷۲ hrs of incubation. The mean expression level of MH۱۶srRNA gene at these concentrations decreased ۰.۸-۱-fold among the isolates compared to the control. Conclusion: Low levels of M. hominis were detected in vaginal samples in Samawah, Iraq. Curcumin could inhibit the growth of M. hominis causing vaginosis. More in-depth and inclusive studies are required to verify the findings.

کلیدواژه ها:

Mycoplasma hominis ، Curcumin ، Fluoroquinolones ، Vaginosis