Research Article: Anti-Alzheimer and antioxidant activities of bacteria isolated from the Persian Gulf sponges

Acetylcholinesterase (AChE) inhibitors are vital in the management of Alzheimer’s disease. This study aimed to identify bacteria isolated from sponge samples collected near Hengam Island and evaluate their acetylcholinesterase inhibitory activity. Sponge samples of Halicona sp. and Axinella sp. were collected by scuba diving. A total of ۱۳۱ bacterial strains were isolated using Marine Zobell agar medium. Bacillus and Vibrio species were the most frequently identified bacteria from the sponge samples. Analysis of the extracted metabolites revealed that four isolates could inhibit acetylcholinesterase activity, with IC۵۰ values between ۲۰۳.۶۰ and ۳۸۵.۶۲ μg/ml. According to the MTT cell proliferation assay, HH ۱۵۱ extract exhibited cytotoxic effects on the HUVEC cell line. The AChE inhibitory extracts also showed DPPH radical scavenging activity between ۷۸.۱۲ μg/mL and ۲۳۷.۵۴ μg/mL. The ۱۶S rRNA sequence analysis showed about ۹۹ to ۱۰۰% homology between HH ۱۲۷, HH ۱۵۱, HA ۳۱۵, and HA ۳۳۸ strains with Vibrio azureus, Streptomyces nigrescens, Bacillus pumilus, and Bacillus subtilis species. These findings provided new candidates of the acetylcholinesterase inhibitors from sponge-associated bacteria for in vivo studies.

کلیدواژه ها:

Alzheimer disease ، Acetylcholinesterase ، Enzyme inhibitors ، Persian Gulf ، Sponge-associated bacteria