Evaluation of Newly Produced Equine Chorionic Gonadotropine (eCG/PMSG) with Effects on Immature Rat Ovaries: A Method Article

Equine Chorionic Gonadotropin (eCG), also known as Pregnant Mare Serum Gonadotropin (PMSG), is widely applied in reproductive biotechnology due to its dual follicle-stimulating hormone (FSH)- and luteinizing hormone (LH)-like activities and prolonged half-life. The classical rat ovarian weight bioassay remains one of the standard methods for assessing PMSG potency. This study aimed to compare the biological activity of PMSG preparations produced by Farnood Dam Kara (Iran) and Syntex (Argentina) in immature Wistar rats. Forty-nine immatures female Wistar rats (~۵۰ g) were randomly assigned into six experimental groups, receiving PMSG at ۱۰, ۲۰, or ۴۰ IU (Farnood or Syntex), and one control group receiving saline. A single subcutaneous injection was administered, and ovarian weights were measured ۶۸ hours later. Both Farnood and Syntex PMSG significantly increased ovarian weight compared with controls (۰.۰۶۳ ± ۰.۰۰۶ g). A dose-dependent response was observed in treatment groups: Farnood increased ovarian weight from ۰.۰۹۰ ± ۰.۰۱۳ g (۱۰ IU) to ۰.۱۳۹ ± ۰.۰۱۹ g (۴۰ IU), while Syntex increased it from ۰.۰۸۷ ± ۰.۰۱۱ g (۱۰ IU) to ۰.۱۳۵ ± ۰.۰۲۰ g (۴۰ IU). Both preparations demonstrated comparable dose-dependent effects, confirming their potency. Newly manufactured PMSG (Farnood) may serve as a reliable and cost-effective alternative to imported formulations.

کلیدواژه ها:

Pregnant mare serum gonadotropin (PMSG) ، Ovarian weight ، bioassay ، Gonadotropin potency ، Reproductive biotechnology