The ANRIL rs۴۹۷۷۵۷۴ Variant is Associated with Rheumatoid Arthritis Susceptibility

Background: As the most common inflammatory arthritis worldwide, rheumatoid arthritis (RA) has been shown in numerous studies to be significantly influenced by long non-coding RNAs (lncRNAs), particularly in terms of disease onset and progression. This research explores the possible association between ANRIL (rs۴۹۷۷۵۷۴) polymorphisms and susceptibility to RA. Methods: This study comprised ۱۵۰ confirmed cases of RA alongside ۱۵۰ matched healthy controls. Genotyping of DNA was carried out using the tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR). Results: Based on multivariate logistic regression, carriers of the AG genotype had a ۷.۱۳‑fold higher risk of developing RA compared to those with the AA genotype. Conversely, the GG genotype was associated with a substantial ۰.۱۸-fold reduction in RA risk compared to AA. Additionally, the ANRIL rs۴۹۷۷۵۷۴ variant was shown to increase RA risk across recessive (OR = ۱.۵۲, ۹۵% CI = ۱.۰۶-۲.۱۹, P = ۰.۰۲۲), dominant (OR = ۲.۴۹, ۹۵% CI = ۱.۵۰-۴.۱۳, P = ۰.۰۰۰۳), and over-dominant (OR = ۸.۱۹, ۹۵% CI = ۴.۰۸۶-۱۶.۴, P < ۰.۰۰۰۰۰۰۱) genetic models. Conclusion: This study established a connection between ANRIL genetic variation and an elevated risk of RA within the Iranian population.

کلیدواژه ها:

Rheumatoid arthritis ، ANRIL Long non-coding RNA ، Tetra-primer ARMS-PCR ، Single Nucleotide Polymorphism