Effects of high-intensity interval training on the number of white blood cells, Myeloperoxidase, lactoferrin, elastase, and lactate in young college men

Purpose: This study aimed to investigate the effects of high-intensity interval training (HIIT) with varying durations on white blood cell count and neutrophil function markers (myeloperoxidase, lactoferrin, and elastase) in young men. Method: Thirty-six healthy, untrained males were randomly divided into four groups: control (n = ۹), ۱۵-second (n = ۹), ۳۰-second (n = ۹), and ۴۵-second (n = ۹) exercise protocols. The training program consisted of six sessions over two weeks, with ۱–۲ days of rest between each session. Each session consisted of four to seven "all-out" Wingate tests (۱۵, ۳۰, or ۴۵ seconds) on a cycle ergometer, separated by ۴-minute recovery periods. Blood samples were collected before and ۴۸ hours after the final session to measure the mentioned variables. Data were analyzed using ANCOVA and paired t-tests at a significance level of p<۰.۰۵. Results: Results showed that HIIT had no significant effect on total white blood cell or neutrophil counts compared with the control group. However, neutrophil function markers responded differently to training duration: myeloperoxidase and elastase increased significantly in all training groups, while lactoferrin decreased, especially in the ۳۰- and ۴۵-second protocols. Conclusion: These findings suggest that longer high-intensity exercise durations may impair neutrophil function by altering the secretion of related enzymes.

کلیدواژه ها:

High-intensity interval training ، Myeloperoxidase ، Lactoferrin ، Elastase