Research Article: Mitochondrial Cyt b and CR differentiation and in silico RFLP markers for Bangladeshi and Vietnam-originated Climbing Perch (Anabas testudineus)

This study employed mitochondrial Cytochrome b (Cyt b) and Control Region (CR) markers to investigate the genetic diversity and population structure of Bangladeshi and Vietnam-origin climbing perch (Anabas testudineus) populations across Bangladesh. A total of ۳۲۰ individuals were collected from ۱۶ sampling sites representing all eight administrative divisions of Bangladesh. Following quality trimming, ۸۱۸ bp of Cyt b and ۷۰۵ bp of CR sequences were analyzed, revealing ۹ and ۲۸ haplotypes, respectively. Analysis of molecular variance (AMOVA) indicated that the majority of genetic variation was attributable to differences among populations (Cyt b: ۹۵.۶%; CR: ۸۹.۵%). Additionally, substantial genetic differentiation was observed between Bangladeshi and Vietnam-origin groups (Cyt b: ۶۷.۵%; CR: ۷۳.۰%). Pairwise Fst values (Cyt b: ۰.۸۱۵; CR: ۰.۸۰۴) confirmed strong differentiation between Bangladeshi and Vietnam-originated groups. Bayesian phylogeny and haplotype network analyses separated Bangladeshi and Vietnam-origin stocks into distinct clades. Shared haplotypes in Dhaka and Chattogram indicated admixture with Vietnam-originated stocks. Several unique haplotypes were identified in both Bangladeshi and Vietnam-originated populations, which may serve as markers for stock identification and broodstock management. In silico RFLP analysis identified seven enzymes (AciI, HaeIII, HhaI, MboI, MspI, RsaI, and StuI) for Cyt b sequences as cost-effective, practical tools for stock identification, while no diagnostic enzymes were found for CR. These findings provide a genetic baseline for conservation and selective breeding, and highlight the urgent need to protect Bangladeshi A. testudineus from admixture with exotic strains.

کلیدواژه ها:

Bangladesh ، Climbing perch ، Control Region ، Cyt b ، mtDNA ، Population structure ، RFLP markers