Hydroxytyrosol protects sperm viability and DNA integrity in human asthenoteratozoospermia during incubation: An experimental study

Background: Asthenoteratozoospermia (AT), marked by poor sperm motility and abnormal morphology, is a major cause of male infertility. During semen handling, AT samples generate high reactive oxygen species (ROS) levels, increasing oxidative stress and sperm damage. Objective: This study investigated the effects of hydroxytyrosol (HT), a natural antioxidant, on sperm quality and ROS levels in AT samples during incubation. Materials and Methods: This experimental study was conducted in ۲ phases. In phase I, ۲۰ AT samples were divided into ۵ groups (۰, ۲۵, ۵۰, ۷۵, and ۱۰۰ μg/mL HT) and incubated for ۳۰, ۴۵, and ۶۰ min to determine optimal conditions based on sperm motility and viability. In phase II, another ۲۰ AT samples were treated with ۲۵ and ۵۰ μg/mL HT for ۳۰ min. Sperm mitochondrial membrane potential, intracellular ROS, DNA fragmentation, and lipid peroxidation were evaluated. Results: Sperm motility decreased after incubation; however, treatment with HT maintained motility, although the difference was not statistically significant. Similarly, sperm viability declined following incubation compared to the baseline. ۲۵ μg/mL HT significantly preserves sperm viability after ۳۰ min compared to ۰ μg/mL HT (p = ۰.۰۲). Incubation significantly increased DNA fragmentation; however, at both ۲۵ and ۵۰ μg/mL HT, DNA fragmentation remained comparable to pre-incubation levels. No significant effects were observed on lipid peroxidation, ROS levels, or mitochondrial membrane potential. Conclusion: Incubation of AT sperm with HT for ۳۰ min preserved sperm viability and reduced DNA damage, suggesting that HT may serve as a beneficial antioxidant in assisted reproductive technologies for men with AT by minimizing oxidative stress-related sperm damage.

کلیدواژه ها:

Abnormal semen ، Antioxidant ، Incubation time ، Oxidative stress ، DNA integrity ، Asthenoteratozoospermia. ، مایع منی غیرطبیعی ، آنتی اکسیدان ، زمان انکوباسیون ، تنش اکسایشی ، یکپارگی DNA ، آستنوتراتوزوسپرمی.