The effect of intratesticular autologous platelet-rich plasma injection on sperm DNA fragmentation in men with oligoasthenoteratozoospermia: An RCT

Background: Oligoasthenoteratozoospermia (OAT) is one of the most common multifactorial causes of male infertility. Platelet-rich plasma (PRP), a biological product, has emerged as a promising regenerative therapy in various fields of medicine, including reproductive health. Therefore, we investigated the effect of PRP administration on sperm DNA fragmentation in OAT men. Objective: The aim was to evaluate the effect of intratesticular injection of PRP on DNA fragmentation index (DFI) in men with idiopathic OAT and assess its potential as a novel therapeutic intervention to enhance sperm DNA integrity. Materials and Methods: This randomized controlled trial study involved ۱۶۰ infertile men diagnosed with OAT without underlying diseases who were referred to the infertility center of Fatemieh Infertility Center, Hamadan, Iran, from July ۲۰۲۳ to July ۲۰۲۴. The participants were randomly assigned to intervention and control groups using a block randomization method. The intervention group received ۱-۲ cc of PRP in each testicle. Sperm parameters and DFI were measured; a hormonal study was also performed before and after the procedure. Results: The results revealed a significant difference in concentration (p = ۰.۰۳۰), progressive motility (p < ۰.۰۰۱), DNA fragmentation (p < ۰.۰۰۱), normal morphology (p < ۰.۰۰۱), and also testosterone levels (p < ۰.۰۰۱) between groups after PRP injection. Conclusion: PRP Injection into the testis appears to be a safe, minimally invasive, and potentially effective therapeutic approach in enhancing sperm quality and reducing DFI in men with OAT.

کلیدواژه ها:

Male infertility ، Oligoasthenoteratozoospermia ، Platelet-rich plasma ، Sperm DNA fragmentation. ، ناباروری مردان ، الیگوآستنوتراتوزواسپرمی ، پلاسمای غنی از پلاکت ، قطعه قطعه شدن DNA اسپرم.